rabbit anti mouse cx43 polyclonal primary antibody  (Cell Signaling Technology Inc)

Journal: Genetics and molecular research : GMR

Article Title: Tiaogan Qingxin Granule treatment increases myocardial connexin 43 expression in a rat model of arrhythmia.

doi: 10.4238/gmr.15027934

Figure Lengend Snippet: Figure 2. Western blots of Cx43 and GAPDH proteins in each group. Lane 1, Sham-operated group. Lane 2, Arrhythmia model group. Lane 3, Wenxin Granule group. Lane 4, Tiaogan Qingxin Granule group.

Article Snippet: The proteins were diluted with 0.9% NaCl to obtain same concentration and same amounts were denatured at 100°C for 3 min. Proteins were then separated by 10% sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE), transferred to PVDF membranes, stored at 4°C for 80 min, and blocked with 5% TBST in skim milk for 1 h. Subsequently, the membranes were treated with rabbit anti-mouse Cx43 polyclonal primary antibody (diluted 1:1,000 in TBST; Cell Signaling Technology, Danvers, MA, US) at 4°C overnight, washed, and incubated with horseradish peroxidase-conjugated secondary antibody (diluted 1:2,000; ZSGB-BIO, Beijing, China) for 1 h at room temperature.

Techniques: Western Blot